Journal: eLife

Article Title: Shore crabs reveal novel evolutionary attributes of the mushroom body

doi: 10.7554/eLife.65167

Figure Lengend Snippet: ( A, B ). Amira-generated 3D reconstructions of the gyri and their calyces. Black arrows indicate the direction of the eyestalk nerve; likewise in panels C–H . ( C ) Ensemble of efferent dendrites in gyrus 2 associated with calyx 1 (Ca1Gy2). The represented area is boxed in panel B . ( D ) Afferent tributary from the olfactory globular tract (olfactory projection neurons) supplying gyrus Ca1Gy1 neuropil (open rectangle in panel A ). ( E ) Smooth oval presynaptic specializations denote the terminals of another olfactory globular tract tributary that exclusively supplies the most proximal gyrus (pGy, rectangle in panel A ), which has no obvious connection to the mushroom body. ( F ) Double-labeling with anti–GAD (yellow) and anti-allatostatin (cyan) of the lateral protocerebrum resolves allatostatin immunoreactivity in the proximal gyrus (rectangle in A and F ), which shows little to no GAD immunoreactivity. ( G, H ) Stacked z-axis projections of Golgi-impregnated tracts, imaged through two successive 50 μm sections showing massive supply by afferent neurons. These reach levels of the lateral protocerebrum that include those at and well beneath the gyriform layer (box I, panel G ), including levels of the calyces (box J in panel G and box K in panel H ). Notably, many efferent neurons relaying information from gyri (panel H ) send their downstream axons from the lateral protocerebrum into these tracts. ( I–K ) Enlargements from regions indicated in G , H , but at sample thicknesses of less than 20 μm to isolate details obscured in flattened optical sections. Scale bars, A–C , F–H , 100 μm; D , E , 50 μm; I–K , 50 μm.

Article Snippet: Antibody , Allatostatin (Ast7; Mouse, monoclonal) , Developmental Studies Hybridoma Bank (DSHB) , CAT#: 5F10; RRID: AB_528076 , 1:100.

Techniques: Generated, Labeling

Journal: eLife

Article Title: Shore crabs reveal novel evolutionary attributes of the mushroom body

doi: 10.7554/eLife.65167

Figure Lengend Snippet:

Article Snippet: Antibody , Allatostatin (Ast7; Mouse, monoclonal) , Developmental Studies Hybridoma Bank (DSHB) , CAT#: 5F10; RRID: AB_528076 , 1:100.

Techniques: Staining, Purification, Electron Microscopy, Software, Microscopy, Light Microscopy

Journal: iScience

Article Title: Gene repression through epigenetic modulation by PPARA enhances hepatocellular proliferation

doi: 10.1016/j.isci.2022.104196

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-UHRF1 , Cell Signaling Technology , Cat# 12387; RRID: AB_2715501.

Techniques: Virus, Recombinant, Chromatin Immunoprecipitation, AST Assay, Immunoprecipitation, shRNA, Luciferase, Software

Journal: Scientific reports

Article Title: Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells.

doi: 10.1038/s41598-019-45514-3

Figure Lengend Snippet: Figure 3. Tissue-specific gene expression analysis by Real-Time PCR. The relative quantities of tissue-specific genes were measured at the mRNA level to assess the final maturation of the terminally differentiated HLCs for both experimental conditions. The results showed an overall trend of higher expression of all markers for the hiPSC-EB + EC-HLCs when compared to the hiPSC-EB-HLCs, with statistically significant higher expression for the albumin, HNF-4α, HNF-4β, CYP1A2, CYP2B6 and UGT1A3. Almost for all the genes tested, both hiPSC-EB-HLCs and hiPSC-EB + EC-HLCs showed a gene expression that was statistically higher than the one observed in human primary hepatocyte (HPH) and human neonatal hepatocyte (HNH). HPH was used as a positive control for mature phenotype, while HNH was used as a control for immature genotype. Results for the differentiated hiPSCs for both conditions were normalized to undifferentiated hiPSCs (experimental negative control). Data presented as mean ± SD (n = 3). *p < 0.05; **p < 0.01; ***p < 0.001.

Article Snippet: We used the following human specific primary antibodies: rabbit anti SOX17 (Santa Cruz, sc-20099; 1:100); mouse anti FOXA2 (Abcam, ab60721); 5 μg ml−1, goat anti Hhex (Santa Cruz, sc-15128; 1:100); mouse anti GATA-4 (Santa Cruz, sc-25310; 1:100); mouse anti AFP (Santa Cruz, sc-166325; 1:100); mouse anti HNF-4α (Santa Cruz, sc-8987; 1:100); goat anti Albumin (Santa Cruz, Santa Cruz, CA, sc-46293; 1:100); mouse anti Cytokeratin 18 (CK-18) (Abcam, ab82254, 5 μg ml−1); mouse anti HNF1-α (Santa Cruz, sc-135939; 1:100); rabbit anti human C-MET (Santa Cruz, sc-10; 1:100), mouse anti human AGXT2L2 (ALT) (Santa Cruz, sc-365670; 1:100), mouse anti human AATM (E-7) (AST) (Santa Cruz, sc-271702; 1:100), mouse anti human AAT (H-7) (A1AT) (Santa Cruz, sc-166018; 1:100), mouse anti HNF-3β (RY-7) (Santa Cruz, sc-101060; 1:100), and Alexa Fluor 488 mouse anti-CD31 antibody (Abcam; ab215911).

Techniques: Gene Expression, Real-time Polymerase Chain Reaction, Expressing, Positive Control, Control, Negative Control

Journal: Scientific reports

Article Title: Generation of fully functional hepatocyte-like organoids from human induced pluripotent stem cells mixed with Endothelial Cells.

doi: 10.1038/s41598-019-45514-3

Figure Lengend Snippet: Figure 4. Tissue-specific marker analysis through immunofluorescence and FACS analysis. Following the differentiation program, terminally differentiated hiPSC-EB + EC-HLCs expressed mature hepatocyte-specific markers, as evidenced by the presence co-staining of (a) ALBUMIN and Alpha-1 Anti-Trypsin (A1AT), (b) ALBUMIN and ALT, (c) ALBUMIN and AST (d) ALBUMIN and CD31, (e) ALBUMIN and HNF-3β. Scale bar 100 µm. (f) FACS analysis for albumin positive cells showed a greater percentage of albumin positive cells in the condition with endothelial compared with the one without (86% vs 59%). (g) Magnified (60X) detail of ALBUMIN/CD31 positive cells showing the rosettes organization of CD31 positive cells interspersed with ALBUMIN positive cells. Scale bar 50 µm.

Article Snippet: We used the following human specific primary antibodies: rabbit anti SOX17 (Santa Cruz, sc-20099; 1:100); mouse anti FOXA2 (Abcam, ab60721); 5 μg ml−1, goat anti Hhex (Santa Cruz, sc-15128; 1:100); mouse anti GATA-4 (Santa Cruz, sc-25310; 1:100); mouse anti AFP (Santa Cruz, sc-166325; 1:100); mouse anti HNF-4α (Santa Cruz, sc-8987; 1:100); goat anti Albumin (Santa Cruz, Santa Cruz, CA, sc-46293; 1:100); mouse anti Cytokeratin 18 (CK-18) (Abcam, ab82254, 5 μg ml−1); mouse anti HNF1-α (Santa Cruz, sc-135939; 1:100); rabbit anti human C-MET (Santa Cruz, sc-10; 1:100), mouse anti human AGXT2L2 (ALT) (Santa Cruz, sc-365670; 1:100), mouse anti human AATM (E-7) (AST) (Santa Cruz, sc-271702; 1:100), mouse anti human AAT (H-7) (A1AT) (Santa Cruz, sc-166018; 1:100), mouse anti HNF-3β (RY-7) (Santa Cruz, sc-101060; 1:100), and Alexa Fluor 488 mouse anti-CD31 antibody (Abcam; ab215911).

Techniques: Marker, Immunofluorescence, Staining